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| Yáñez,Romina; Bastías,Roberto; Higuera,Gastón; Salgado,Oscar; Katharios,Pantelis; Romero,Jaime; Espejo,Romilio; García,Katherine. |
| Background The surveillance of Vibrio parahaemolyticus in the Chilean coast has been mainly performed by multiplex PCR amplification of three different hemolysin genes, which are specie-specific virulence factors. These genes are also employed in the determination of V. parahaemolyticus pathogenic load in seafood and for characterization of pathogenic strains associated to diarrhea cases in human. During environmental surveillance that we performed every summer, we occasionally observed a thermolabile hemolysin (tlh) PCR product of a slightly smaller size than expected, which was coincident with low loads of V. parahaemolyticus in the environment. In order to understand this observation, we probed the specificity of tlh primers for the detection of V.... |
| Tipo: Journal article |
Palavras-chave: Hemolysin; Multiplex PCR; Pathogen surveillance; Virulence factor; Vibrio parahaemolyticus. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600012 |
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| Espinosa,Ivette; Báez,M; Vichi,J; Martínez,Siomara. |
| Pasteurella multocida is an important veterinary pathogen that causes a range of animal diseases, including fowl cholera, hemorrhagic septicemia in cattle and atrophic rhinitis in swine. P. multocida is generally recognized as a secondary invader contributing substantially to respiratory diseases in pigs by aggravation of lung lesions. Five capsule serogroups are routinely identified in P. multocida (A, B, D, E, and F) and each is generally associated with, but not completely restricted, to a specific host. A total of 16 isolates of Pasteurella multocida capsular genotype A and two isolates capsular genotype D were characterized for their susceptibilities to 10 antibiotics and the presence of four genes for virulence factors associated to adherence. The... |
| Tipo: Journal article |
Palavras-chave: P. multocida; Virulence factor; PCR; Antimicrobial susceptibility. |
| Ano: 2012 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2012000300003 |
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| Tokano,Dorismey Vieira; Kawaichi,Marisa Emiko; Venâncio,Emerson José; Vidotto,Marilda Carlos. |
| The aim of this work was to isolate, clone and characterize the iron uptake gene iutA from avian pathogenic E. coli (APEC). The iutA gene was isolated from the strain APEC 9, serotype O2:H9, which was cloned in the expression vector pET101/D-TOPO. The gene of 2.2 Kb was sequenced (AY602767, which showed high similarity to the iutA gene from three plasmids, two from APEC, pAPEC-02-ColV (AY545598.4) and pTJ100 (AY553855.1), and one from a human invasive E. coli strain, the pColV K30. The recombinant protein IutA was over expressed in E. coli BL21(DE-3) and was solubilized with urea and purified by Ni-NTA column. This method produced a relatively high yield of r-IutA of approximately 74kDa, which was used to produce the antibody anti-IutA. This anti-IutA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Avian Escherichia coli; Iron uptake; Aerobactin receptor; Virulence factor; IutA gene. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300006 |
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| Gomes,Tânia A.T.; Elias,Waldir P.; Scaletsky,Isabel C.A.; Guth,Beatriz E.C.; Rodrigues,Juliana F.; Piazza,Roxane M.F.; Ferreira,Luís C.S.; Martinez,Marina B.. |
| ABSTRACT Most Escherichia coli strains live harmlessly in the intestines and rarely cause disease in healthy individuals. Nonetheless, a number of pathogenic strains can cause diarrhea or extraintestinal diseases both in healthy and immunocompromised individuals. Diarrheal illnesses are a severe public health problem and a major cause of morbidity and mortality in infants and young children, especially in developing countries. E. coli strains that cause diarrhea have evolved by acquiring, through horizontal gene transfer, a particular set of characteristics that have successfully persisted in the host. According to the group of virulence determinants acquired, specific combinations were formed determining the currently known E. coli pathotypes, which are... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Diarrhea; Pathogenic mechanisms; Virulence factor; Epidemiology. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000500003 |
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| Kanabata,B; Menck-Costa,MF; Souza,M; Justino,L; Rangel,IG; Kobayashi,RKT; Nakazato,G; Baptista,AAS. |
| ABSTRACT Avian cellulitis causes significant losses to the poultry industry. Avian-pathogenic Escherichia coli (APEC) is the etiological agent of that disease. This microorganism has zoonotic potential and may act as reservoir of antimicrobial-resistance genes. In this context, the production of extended-spectrum B-lactamase (ESBL) is one of the main antimicrobial resistance mechanisms. The objective of this study was to determine the production of ESBL in an Escherichia coli (E. coli) strain isolated from avian cellulitis lesions. Twenty-two E. Coli isolates were harvested from cellulitis lesions in chicken carcasses in a commercial processing plant. Isolates were then submitted to virulence genotypic profile (iutA, hlyF, iss, ironN, ompT) analysis,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: APEC; Avian cellulitis; ESBL; MDR; Virulence factor. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300326 |
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| Esteves, Kevin; Mosser, Thomas; Aujoulat, Fabien; Hervio-heath, Dominique; Monfort, Patrick; Jumas-bilak, Estelle. |
| Vibrio parahaemolyticus and Vibrio cholerae are ubiquitous to estuarine and marine environments. Thes two species found in Mediterranean coastal systems can induce infection in humans. Environmental isolates of V. cholerae (n = 109) and V. parahaemotycius (n = 89) sampled at different dates, stations and water salinities were investigated for virulence genes and by a mutilocus sequence-based analysis (MLSA). V. cholerae isolate were all ctxA negative and only one isolate of V. parahaemolyticus displayed trh2 gene. Most Sequence Types (ST) corresponded to uique ST isolated at one date or one station. Frequent recombination events were detected among different pathogenic species, V. parahaemotyticus, V. cholerae, Vibrio mimicus, and Vibrio metoecus.... |
| Tipo: Text |
Palavras-chave: Vibrio; Human pathogens; French coastal lagoons; Phylogeny; Recombination; Multi-locus sequence analysis; Virulence factor. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00274/38528/37039.pdf |
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| Rigobelo,E.C.; Gamez,H.J.; Marin,J.M.; Macedo,C.; Ambrosin,J.A.; Ávila,F.A.. |
| One hundred seventy-three Escherichia coli strains isolated from calves from northwestern São Paulo State, having diarrhea were examined for the production of thermolabile (LT) and thermostable (ST) enterotoxins and for the presence of virulence factors associated with bovine colibacillosis. Eighty-five (49.1%) of the E.coli strains produced toxins; 53 isolates were detected as producing STa toxin, and 9 also produced LT toxin. By PCR, 23 isolates were shown to harbor only the LT-II gene. Nine (5.2%) isolates harbored Shiga toxin genes: four carried the stx2 gene, four the stx1 gene and one carried both. Three of the isolates showing stx1 also carried the eae gene. Among the E. coli isolates examined for susceptibility to 10 antimicrobial agents,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calf; Diarrhea; Escherichia coli; Virulence factor; Antimicrobial agent. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300003 |
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